Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the

Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell
Joachim Frank
Publisher: Springer
Number Of Pages: 455
Publication Date: 2006-12-18
ISBN-10 / ASIN: 038731234X
ISBN-13 / EAN: 9780387312347
Binding: Hardcover
Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with special emphasis on the problems presented limitations of data collection in the transmission electron microscope. In addition to chapters that are applicable to 3D reconstruction in all fields of science, such as radiological imaging in medicine and electron tomography in materials science, Electron Tomography also focuses on specimen preparation and imaging unique to biological electron microscopy.
This extensively revised second edition updates key contributions on the mathematics of 3D reconstruction, and includes new topics such as automated tomography, frozen sectioning of cells, and the interpretation of density maps through methods of fitting, docking, denoising, and segmentation. Each chapter is a self-contained treatise a world expert in the author’s field of research, resulting in an indispensable resource and companion for laboratories that practice electron tomography or seek to implement electron tomography as a tool for visualization of cells and cell components.
Key Features
Presents the mathematical background and working methods for three-dimensional reconstruction from projections
Takes the reader from biological specimen preparation to three-dimensional images of the cell and its components
Revised and updated extensively from the first edition published in 1992
The definitive work in the field written leading international experts
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I have to agree with you Bernd1964
The big question is: why were microwaves classified as ‘dangerous’in 60s even in low field intensities by the army and are now used for mass communication deliberately? To me it seems the greed for profits has overtaken every health caution. Our civilization is in its end times, we take no cautions and have no responsiblities anymore, we just live for the moment. We don’t think about tomorrow.
i know this is true